Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

First report of Lasiodiplodia pseudotheobromae causing trunk cankers in Citrus reticulata in Pakistan.

Abstract

In summer of 2015, symptoms of cankers, necrosis and gum exudates were observed in the trunk bark of a citrus cultivar (Citrus reticulata, Kinnow) were collected from 10 orchards of C. reticulata in Sargodha, Bhulwal, Kot Momin, Faisalabad and Toba Tek Singh in Pakistan. Disease occurrence was approximately 40% in the visited fields. A total of 25 symptomatic cankered, necrotic, and gum-filled exuded bark samples were cut to 2 to 4 mm wide at lesion margins for pathogen isolation and identification. Based on morphological characters, DNA sequencing and pathogenicity tests, the canker isolates from Pakistan were identified as Lasiodiplodia pseudotheobromae. This is thought to be the first report of L. pseudotheobromae causing trunk cankers in C. reticulata in Pakistan.