Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

First report of barley yellow dwarf virus PAV in uncultivated grasses of Pakistan.

Abstract

Barley yellow dwarf virus PAV (BYDV PAV; icosahedral virions; ss(+)RNA genome; genus Luteovirus; family Luteoviridae) is a phytopathogen causing yellow dwarf disease, which is epiphytotic to almost all cereal growing regions. Apparently asymptomatic and symptomatic (reddening, yellowing and stunted plant growth) leaf samples attributed to luteovirid infection were collected in winter (November, 2011) from uncultivated grasses, i.e. Johnsongrass (Sorghum halapense) and Italian ryegrass (Lolium multiflorum), from Pothohar region near federal capital area of Pakistan. Along with the presence of symptoms, Enzyme-Linked Immunosorbent Assay (ELISA) with a BYDV-PAV reagent set, according to the manufacturer's protocol, confirmed BYDV-PAV infection. ELISA results showed 70% disease incidence on symptomatic plants, while 30% were negative for BYDV-PAV. This results was further confirmed by RNA sequencing. BYDV-PAV was first reported in Pakistan with serological confirmation in 1997, and was found to the most prevalent strain in the cereal crop. This is thought to be the first report of BYDV infecting uncultivated grasses in Pakistan.