Potato spindle tuber viroid detected in seed of uncultivated Solanum anguivi, S. coagulans and S. dasyphyllum collected from Ghana, Kenya and Uganda.
In June 2018, 98 seed samples (100 seeds per sample) of the solanaceous species, namely Solanum anguivi, S. anomalum, S. cerasiferum, S. coagulans [Solanum melongena]>, S. dasyphyllum, S. incanum, S. macrocarpon and S. virginianum, were tested for Potato spindle tuber viroid (PSTVd). RNA was extracted from the seeds using a magnetic bead extraction method and tested by real-time RT-PCR. Eight of the samples tested positive using this assay: five samples of S. anguivi, two of S. dasyphyllum and one of S. coagulans. The resulting PCR products were sent for sequencing, PSTVd was identified and sequences added to GenBank. The available sequence for the related herbarium and seed samples shared 98-99% homology. This is the first report of a Pospiviroid being detected in S. anguivi, S. coagulans and S. dasyphyllum, confirming the potential for pospiviroids to be distributed through non-commercial seed.