First report of Groundnut bud necrosis virus infecting Phalaenopsis in India.
During 2016-17, Phalaenopsis plants grown at the National Research Centre for Orchids (Sikkim, India) were observed with symptoms including mild chlorotic spots, mild-mosaic and dark-green patches with light-green/chlorotic margins. Twelve symptomatic and five non-symptomatic samples were collected and transmission electron microscopy revealed the presence of tospovirus-like particles measuring 80-110 nm in diameter in symptomatic samples. Based on the results of DAC-ELISA, pathogenicity, RT-PCR and sequence analysis, the causal agent was identified as Groundnut bud necrosis virus (GBNV). This is thought to be the first report of GBNV infecting Phalaenopsis in India and the world.