First report of garlic virus C infecting garlic in India.
During February 2018, symptoms such as mosaic, leaf curling, and stunting were observed on 8-week-old garlic (Allium sativum) cv. G-324, at the experimental plot of ICAR-DOGR, Pune, Maharashtra, India. It indicated infection of garlic viruses. The incidence of symptoms was 20% of the garlic plants. Leaves of six randomly selected symptomatic plants of garlic accession G-324 were collected. Total RNA was extracted individually from the leaves of six garlic plants and followed by cDNA synthesis. Garlic leaf samples were then tested through RT-PCR for garlic viruses such as Onion yellow dwarf virus (OYDV), Leek yellow stripe virus (LYSV), Garlic common latent virus (GarCLV), Garlic virus A (GarV-A), Garlic virus C (GarV-C), Garlic virus D (GarV-D), and Garlic virus X (GarV-X) using primers available in the NCBI database. All the samples were found positive for GarV-C, GarV-A, GarV-D, GarV-X, GarCLV, and OYDV. Additionally, two samples were found positive for LYSV. The CP gene of GarV-C was amplified. BLAST analysis of the CP gene sequence revealed 81% nucleotide (JN019814.1) and 86% amino acid (AEV51814.1) sequence identity with a GarV-C isolate from Australia. This is thought to be the first report of GarV-C from India.