First report of Tomato brown rugose fruit virus infecting sweet pepper in Italy.
In January 2020, approximately 85% of a red sweet pepper (Capsicum annuum) crop in a greenhouse located in Ragusa province (Sicily, Italy) showed virus-like symptoms. Symptoms consisted of a slight mosaic and discoloration of young leaves, vein clearing on young leaves, browning of the stem with strong necrosis located in the intersection of the secondary branches, partial necrosis of the vegetative apex and marbling, mosaic and distortion of the fruits. In 2019, the same greenhouse was cultivated with tomato, which had been removed due to extensive infection caused by Tomato brown rugose fruit virus (ToBRFV). Thirty plants (15 diseased and 15 asymptomatic) were collected for subsequent analysis. Based on the results of RT-PCR and BLAST analysis of the obtained nucleotide sequences, as well as symptomatology, the causal agent was identified as ToBRFV. This is thought to be the first report of ToBRFV on pepper in Italy and in Europe.