First report of grapevine Pinot gris virus in Lebanon and the Middle East.
A total of 108 grapevine samples representing 14 cultivars (Black Magic, Superior Seedless, Midnight Beauty, Black Pearl, Red Globe, Crimson, Vermentino, Marselan, Chardonnay, Petit Verdot, Cabernet Sauvignon, Merlot, Pinot noir, Syrah) were collected randomly from nine vineyards located in Bekaa Valley of Lebanon. Samples were analysed by RT-PCR for the presence of Grapevine Pinot gris virus (GPGV) using two sets of primer pairs (DetF/DetR) targeting the movement protein (MP) and coat protein (CP) genes and primers (GPGVRepF/GPGVRepR) partially covering the RNA-dependent RNA polymerase (RdRp) domain of the GPGV replicase gene. Results showed that two Black Magic and four Superior Seedless table grape cultivars, and four Vermentino and four Marselan wine grape cultivars were infected with GPGV. None of the infected vines showed disease symptoms. Infection with GPGV was found in single or multiple infections with Grapevine virus A and Grapevine fleck virus. This is thought to be the first detection of GPGV in Lebanon and the Middle East.