Presence of viruses in the population of grapevine cultivar "Prokupac" (Vitis vinifera L.) in Rasina district, Serbia.
Grapevine cultivar "Prokupac" is officially recognized as Serbian autochthonous variety, It has a long history, tracing to the Middle Ages. This cultivar is used to be the most widespread variety in this part of Balkans and best-rated domestic Serbian wine at international markets. Survey of virus infection incidence in grapevine nurseries, was conducted at 14 location of Rasina district (Republic of Serbia), during 2018. The survey was conducted by Agricultural Service Krusevac, as authorized institution with a professional capacity included into a national phytosanitary system, for implementation of the system of control of prevention of fruit tree and grapevines pathogens (including export and import). It also controls the presence of pathogens in main nurseries. Total number of 17 samples was tested on presence of four viruses using ELISA: Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated viruses 1, 2 and 3 (GLRaV-1, GLRaV-2, GLRaV-3). The presence of GLRaV-1 was confirmed in one tested sample, and the presence of GLRaV-1, GLRaV-2, GLRaV-3 were also confirmed in one tested sample. Conducted investigation indicates deteriorated viral sanitary status of cultivar "Prokupac" and necessity for intensifying the clonal and sanitary selection program.