In vitro grafting of Psidium guajava in Psidium cattleianum for the management of the Meloidogyne enterolobii.
Guava is one of the most important tropical fruit crops; however, its cultivation has been affected by the prevalence of the nematode Meloidogyne enterolobii, which leads to a large-scale reduction in the production of fruits and restricts new plantations in the contaminated areas. The discovery of the resistance of Psidium cattleianum to this nematode has increased the possibility of its selective use as rootstock for guava; a few studies have worked on the graft; however, the rootstock exhibits a high incompatibility with the commercial varieties of guava. Therefore, the present study aimed to develop an in vitro-grafting technique of guava scions under rootstocks of P. cattleianum obtained using an in vitro seed germination method. The successful in vitro-grafting was performed using semisolid MS1/2 (Murashige and Skoog) culture medium with 0.5 mg L-1 of Benzyladenine. P. cattleianum with approximately 3 cm length was used as rootstocks, and apical guava stems with 0.5 cm length were used as scions. The grafting procedure used was wedge grafting. To increase the success rate of in vitro-grafting, we used a sterile paper moistened with sterilized distilled water to prevent dehydration. The use of MS1/2 and the wedge grafting resulted in the overall success rate of 30% for the graft between P. cattleianum and Psidium guajava. In conclusion, this is the first report of successful in vitro-grafting between guava and P. cattleianum.