Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

First report of Grapevine Pinot gris virus on grapevines in Armenia.

Abstract

Grapevine Pinot gris virus (GPGV) is a member of the genus Trichovirus in the family Betaflexiviridae. It was first described on Italian grapevine (Vitis vinifera) cv. Pinot gris, in which it provoked a disease known as grapevine leaf mottling and deformation (GLMD). In 2018, 17 grapevine cultivars (Ararati, Arevabuyr, Arevar, Armenia, Eraskheni, Garan Dmak, Itsaptuk, Kishmish White, Masis, Meghrabuyr, Mskhali, Rkatsiteli, Shahumyani, Tokun, Vani, Vardaguyn Yerevani, and Voskehat) were selected in economically important Armenian vineyards of Yerevan city and Merzdavan village, Armenia for GPGV detection. The grapevines were selected based on possible GLMD symptoms. The plants were tested for the presence of GPGV by reverse transcription polymerase chain reaction. GPGV was detected in cultivars Ararati, Arevabuyr, Eraskheni, Garan Dmak, Itsaptuk, and Mskhali based on the amplification of gene fragments specific to the MP-CP and RdRp genome regions. High-throughput sequencing revealed the presence of GPGV in mixed infection with Grapevine fleck virus, Grapevine leafroll-associated virus 1, Grapevine fanleaf virus and Grapevine virus A in the sample. Data showed a high prevalence of GPGV, roughly 35%, in Armenia.