First report of turnip yellows virus on cabbage in Serbia.
Cabbage (Brassica oleracea var. capitata) is a cruciferous vegetable consumed worldwide and is used in traditional medicine. In October 2018, during a survey to determine the presence of viral diseases in cabbage, virus-like symptoms were observed on cabbage cv. Srpski melez growing in Serbia. Disease incidence was estimated at 40%. Plants exhibiting symptoms of stunting and purpling of the leaves were collected and tested by commercial double-antibody sandwich (DAS)-ELISA diagnostic kits against Turnip yellows virus (TuYV), Turnip mosaic virus, and Cauliflower mosaic virus. Out of 20 samples tested, 15 were positive for TuYV and negative for the other tested viruses. The aphid transmissibility of the selected serologically positive sample (364Cb) was tested using Myzus persicae as the vector. Total RNAs were extracted from all 15 ELISA-positive cabbage samples using an RNeasy Plant Mini Kit and tested by conventional reverse transcription PCR using the OneStep RT-PCR Kit with specific primers TuYVorf0F/TuYVorf0R. This is thought to be the first report of Turnip yellows virus on cabbage in Serbia.