First report of tomato chlorosis virus infecting cucumber in Brazil.
Cucumber plants (Cucumis sativus) exhibiting chlorosis and mottling symptoms on young leaves were found in a commercial greenhouse, in the county of Oleo, State of São Paulo, Brazil, in February 2019. The crop was highly infested by whiteflies. To identify the whitefly species in the greenhouse, total DNA was extracted from 10 whiteflies. The whiteflies were identified as Bemisia tabaci Mediterranean species (MED, also known as Q biotype) based on the RFLP analysis and BLASTn search of the amplicon sequence. The causal pathogen was isolated and identified as Tomato chlorosis virus based on the complete nucleotide sequence. This is thought to be the first report of Tomato chlorosis virus infecting cucumber plants in Brazil.