First record of Ranunculus white mottle virus from Australia.
In 2004, a vein yellowing disease of soil-grown greenhouse Capsicum was observed in southern Australia. Symptoms were similar to those of pepper yellow vein disease, a suspected viral disease of Capsicum transmitted by soilborne Olpidium species. Disease symptoms were apparent on young plants and faded as the plant matured. In 2008, the disease was again detected in the region and was graft-transmitted to four Capsicum plants which developed vein-yellowing symptoms. This isolate (2155) and a sample lyophilised in 2004 (isolate 1631) were tested by RT-PCR using the OP1 and OP2 primers specific for RNA-1 of the genus Ophiovirus. RT-PCR amplicons of the expected size (136 bp) were obtained from the suspected virus-infected samples but not from healthy capsicum. The causal pathogen was identified as Ranunculus white mottle virus based on the nucleotide sequence analysis. This is thought to be the first report of Ranunculus white mottle virus infecting Capsicum in Australia.