First report of barley yellow dwarf virus in maize in Tunisia.
Barley yellow dwarf virus (BYDV) was identified on barley in Tunisia by Najar et al. (2017). No data is currently available on whether this virus is present on maize (Zea mays). A field survey was conducted during spring of 2018 in the northeastern region (El Alia) of Tunisia. A total of 140 samples were randomly collected from five maize fields and tested by tissue-blot immunoassay using antiserum against BYDV. Fifty-two maize samples (37%) were positive for BYDV. In parallel, 38 out of 52 (73%) maize samples tested by DAS-ELISA using a polyclonal antibody raised against BYDV-PAV (Bioreba) were positive, suggesting a prevalence of BYDV-PAV in maize in Tunisia. The occurrence of BYDV was confirmed by RT-PCR and sequence comparison to known isolates. This is thought to be the first report of BYDV-PAV on maize in Tunisia.