Invasive Species Compendium

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Abstract

Short-term in situ effects of insulin on ion transport in gill and kidney of freshwater climbing perch (Anabas testudineus Bloch).

Abstract

The presence and transport of ions within and between the cells are critical in the physiology of cells. Despite the metabolic actions of insulin on their respective osmoregulatory and metabolic organs, the actions of these hormones on ion transport in gills and kidney are not yet well understood in fish. We therefore examined the in situ effects of insulin on membrane-bound and mitochondrial ion transporters in the freshwater climbing perch, Anabas testudineus Bloch. To achieve this goal varied doses (10-9, 10-8, 10-7 M) of insulin were infused in these fish for 20 min and the specific activities of ion-specific ATPases were analyzed. Insulin infusion (10-8 M) produced significant increases in the activities of all tested transporters in the gills except mitochondrial Ca2+-ATPase activity. Similarly, in kidney, Na+, K+-ATPase activity showed a significant decrease whereas all other ATPases showed dosedependent changes. However, a direct action of insulin on ion transport was found in both gills and kidney tissues, as evident in the activity patterns of ion-specific ATPases. In short, the results point to a vital role of insulin in ion transport in both gills and kidney tissues of climbing perch.