A molecular detection and identification method for papaya mealybug (Paracoccus marginatus), the first recorded invasive pest in Fujian Province of China.
This paper provides information on the development of a molecular detection and identification method for the papaya mealybug (Paracoccus marginatus), the first recorded invasive insect pest in Fujian, China. The molecular identification of P. marginatus was carried out based on 28S rDNA molecular markers. It was shown that the 28S rDNA species-specific primers had ideal and stable specificity for P. marginatus and can be used to identify P. marginatus accurately. A rapid molecular detection technique for P. marginatus was established based on the species-specific PCR method. The technology had the characteristics of accuracy, rapidity, sensitivity and simplicity. Results indicated that the rapid detection technique could be useful in quarantine at ports, in pest detection and in monitoring during transportation of pawpaws and other fruit tree seedlings as well as flowers.