First report of Diaphorthe masirevicii causing leaf blight of Gloriosa superba in India.
Leaf blight disease was observed on Gloriosa superba during surveys performed in Mysore, Karnataka, India. The first symptoms of infection were small (2-5 mm diameter), circular to oval, light brownish spots, surrounded by a yellow halo. Leaf spots occured on the leaf tips, margins and midribs of the leaves, enlarging to form spots with concentric rings. Embedded in the necrotic tissues were black fruiting bodies of a fungus. These symptoms also appeared later on the stem. The causal pathogen was isolated and identified as Diaporthe masirevicii based on morphological, molecular and pathogenicity analyses. The obtained sequences were deposited in GenBank as accession numbers MF682435 (ITS), MF668289 (Β-tubulin) and MF668290 (TEF). This is thought to be the first report of leaf blight caused by D. masirevicii on G. superba in Karnataka, India.