Effect of Bursaphelenchus xylophilus infestation on antioxidant system of Pinus massoniana.
To reveal the effect of the pine wood nematode (B. xylophilus) on the antioxidant system of P. massoniana, changes in proline content, antioxidant enzyme activity and related gene expression in tree needles infected by the nematode were studied. Pine wood nematode infestation resulted in a significant decrease in proline content, superoxide dismutase, copper-zinc superoxide dismutase and peroxidase activity, and proline-rich arabinogalactan. The coding genes for protein and peroxidases were also down-regulated. With increases in the invasive pest, the expression of the peroxidase gene in P. massoniana needles increased, while the expression in the stems and shoots weakened, leading to anti-oxidation. The enzyme activity decreased, and the pest resistance of P. massoniana was weakened. The results of the study initially revealed changes in the antioxidant system during the final wilt death of P. massoniana caused by the pine wood nematode infestation, and the corresponding prevention and control of pine wood nematode diseases in forestry production. The strategy provides a theoretical basis.