Detection and characterisation of a phytoplasma associated with cucumber (Cucumis sativus) regional yellows disease in Iran.
A phytoplasma was detected in cucumber (Cucumis sativus), exhibiting regional yellows symptoms in leaves, stem and fruits, that was grown in the greenhouse near Tehran (Iran). Since this is a previously undescribed disease, the name cucumber regional yellows have been tentatively assigned to it. Based on in silico RFLP and phylogenetic analysis of PCR-amplified 16S rDNA sequences, the phytoplasma associated with regional yellows disease was identified as a new member of phytoplasma 16S rRNA group VI (16SrVI-A) with closest relationships to zucchini phyllody phytoplasma (KP119494). According to our results, cucumber regional yellows phytoplasma could be designated as a subgroup VI-A.