First report of Tomato spotted wilt virus infecting Peperomia obtusifolia in South Korea.
In July 2018, virus-like symptoms including mottle and yellowing followed by vein clearing on leaves were observed in P. obtusifolia (cv. Shinholly) plants in a greenhouse in Geyongnam province, South Korea. Approximately 20% of peperomia plants (from a total of 3 000 plants) in the greenhouse showed virus-like foliar symptoms. To identify the causal virus, four symptomatic leaf samples of P. obtusifolia plants were analysed by transmission electron microscopy (TEM) of leaf dip preparations. Based on the results of TEM, double-antibody sandwich-ELISA test, reverse transcription polymerase chain reaction assay and nucleotide sequence comparisons, the causal agent was identified as Tomato spotted wilt virus (TSWV). This is thought to be the first report of TSWV in P. obtusifolia in South Korea.