The first report of animal genotypes of Cryptosporidium parvum in immunosuppressed and immunocompetent humans in Slovakia.
The aim of our study was to determine species and genotypes of Cryptosporidium in patients suffering from immunosuppressive illnesses, but also in immunocompetent patients suffering from diarrhoea. A total of 80 samples of faeces were collected from both immunosuppressed and immunocompetent patients. The immunosuppressed patients (65 samples) - 35 adult patients (group A) and 30 children (group B) were hospitalized at the Clinic of Oncohemathology. Samples from immunocompetent humans (15 samples, group C) were taken from patients with clinical signs of acute diarrhoea. With the use of molecular methods targeting the 60 kDa glycoprotein (GP60) gene region, we have identified multiple genotypes of Cryptosporidium. parvum and Cryptosporidium. hominis in immunocompromised, but also in immunocompetent individuals (C. hominis IbA10 G2, IeA12 G3T3; C. parvum IIaA10 G1R1, IIaA11 G2R1, IIaA12 G2R1, IIaA13 G1R1, IIaA14 G1R1, IIaA14 G2R1, IIaA17 G1R1 and IIaA18 G1R1). This is the first report of the occurrence of genotypes IIaA10 G1R1, IIa12 G2R1 and IIaA18 G1R1 in human hosts.