Synthetic blend of larval frass volatiles deter oviposition of the box-tree moth (Cydalima perspectalis).
Volatile stimuli emitted by an intact or herbivore-occupied host plant, non-host plants or the herbivore itself can have all influence final decision of females about where to lay eggs. Volatile substances surrounding larval excreted frass of the invasive box tree moth (Cydalima perspectalis Walker) were collected and the physiological activity was investigated by coupled gas chromatographic-electroantennographic detection. Based on structure elucidation, two aromatic derivates and one terpene alcohol have been identified to be physiologically active on the antennae of the adults: guaiacol, (±)-linalool and veratrol. In all compounds, antennal responses were found to be dose-dependent with EAG amplitudes being the highest at the highest dose levels. Single sensillum recordings on mated female antennae revealed that these frass compounds triggered 22 percent of the tested olfactory sensory neurons housed in trichoid sensilla. Behavioral bioassays indicated that the blend of these compounds had an oviposition deterrent effect on conspecific females.