First report of bacterial spot of tomato caused by Xanthomonas perforans in Mississippi.
In June 2017, leaf spot symptoms were observed on tomato (Solanum lycopersicum) plants in fields within Jasper and Smith counties in Mississippi, USA. Leaves displayed necrotic, circular and irregular lesions. Twenty symptomatic leaves were collected in July 2017 from single (Amelia and 7631) and mixed tomato cultivars in 3 grower fields. Necrotic lesions were also observed on the rachises, peduncles, sepals and petiolules. The causal pathogen was isolated and identified as Xanthomonas perforans based on physiological, biochemical, molecular and pathogenicity analyses. The hrcN, fusA, gapA, gltA, gyrB, lacF and lepA sequences of one representative isolate were deposited in the National Center for Biotechnology Information's GenBank database under accession numbers MH249602, MH550118, MH550119, MH550120, MH550121, MH550122 and MH550123, respectively. This is thought to be the first report of bacterial spot of tomato caused by X. perforans in Mississippi, USA.