First report of grapevine rupestris stem pitting-associated virus, hop stunt viroid, and grapevine yellow speckle viroid 1 infecting grapevine (Vitis vinifera) in Bosnia and Herzegovina.
In Bosnia and Herzegovina (BiH) there is only scarce information on causal agents of grapevine leafroll and infectious degeneration disorders. Next-generation sequencing (NGS) allows the detection of virus nucleic acids in a single plant or in composite samples, facilitating the identification and the discovery of grapevine viruses. This paper presents the results of a study conducted to screen the viruses infecting grapevine (Vitis vinifera) in BiH using NGS technologies and to confirm the presence of the detected viruses by targeted reverse transcription polymerase chain reaction. Analysis of the NGS reads resulted in the identification of Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine Pinot gris virus (GPGV), Hop stunt viroid (HpSVd), and Grapevine yellow speckle viroid 1 (GYSVd-1). GPGV has been previously reported in one of the pooled samples from the Derventa location in BiH, whereas GRSPaV, HpSVd, and GYSVd-1 had never been reported previously. This is thought to be the first report of GRSPaV, HpSVd, and GYSVd infecting grapevine in BiH.