First report of Hibiscus chlorotic ringspot virus in Turkey.
Infected leaves of were collected from eight different plants of Hibiscus rosa-sinensis (rose of China) and stored at -80°C until further analyses. For RT-PCR, a pair of primers specific to parts of the coat protein (CP) gene of Hibiscus chlorotic ringspot virus (HCRSV) were designed (AK-1 HCRSV-F 5'-AAGAGAGCAGCCAATAGA-3' and AK-2 HCRSV-R 5'-GAAGAAGAACAAGAAGCGA-3'), based on the complete genome sequence of HCRSV (GenBank Accession No. NC_003608). Total RNA was isolated and RT-PCR was done. As expected, a 759 bp DNA fragment corresponding to the partial CP gene was amplified from all samples. Based on the sequence analysis and phylogenetic studies, the pathogen was identified as HCRSV. This is thought to be the first report of HCRSV in Turkey.