Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

First report of potato cyst nematode Globodera pallida infecting potato (Solanum tuberosum) in Kenya.

Abstract

During a nationwide survey conducted in 2016, G. pallida was detected in Kenya at an altitude of 2 349 m above sea level in Nyandarua County. Cysts were extracted from a 200 cm3 soil sample following EPPO diagnostic protocol (EPPO 2013a), and then handpicked under a stereo microscope. The potato cyst nematodes recovered showed morphometric characteristics of G. pallida and are reported in this paper. For further studies, the Nyandarua field was resampled in February 2017 to collect additional soil samples and confirm the occurrence of G. pallida. Results are presented of DNA amplification performed from 14 cysts and 25 juveniles using the multiplex-PCR method and the ITS1-5.8S-ITS2 regions. This first report of G. pallida in sub-Saharan Africa has paramount phytosanitary and regulatory implications for potato growers and traders, national extension services, and policy makers in Kenya and the surrounding region.