Dothistroma spp. in western Ukraine and Georgia.
Dothistroma needle blight (DNB) is among the most serious foliar diseases affecting Pinus spp. globally. Infected needles were collected from potential host species in four locations in western Ukraine and in four locations in eastern Georgia during spring-summer 2015 to update the knowledge on pathogen distribution in these countries. Dothistroma spp. were detected using isolation, sequencing and species-specific priming (SSPP) PCR. Two new hosts for Dothistroma spp. were recorded in western Ukraine: D. septosporum on Pinus nigra var. australica and D. pini on P. nigra var. mollet. D. septosporum was found on 15-year-old P. strobus in western Ukraine. New hosts for D. septosporum were recorded in Georgia on 5- to 10-year-old naturally regenerated P. sylvestris var. hamata and on 40- to 50-year-old P. ponderosa trees. D. pini was found for the first time in Georgia on 30- to 40-year-old P. nigra trees. The work confirmed the presence of both D. septosporum and D. pini in western Ukraine and Georgia, and demonstrated new hosts for both Dothistroma species.