First report of avocado sunblotch viroid (ASBVd) naturally infecting avocado (Persea americana) in Greece.
During the 2016 growing season, fruits exhibiting depressed, greenish, yellow-white craters, which are typical symptoms of avocado sunblotch disease, were spotted on a single tree in an orchard located in the province of Chania, Crete. No symptoms were observed on the leaves or shoots of the tree nor on any other tree in the orchard. The causal agent of sunblotch disease is Avocado sunblotch viroid (ASBVd, family Avsunviroidae). RT-PCR and northern blot analysis were carried out and confirmed the presence of the viroid in the symptomatic tree. This is thought to be the first report of ASBVd in Greece.