First report of cyst nematode (Heterodera iri) in Ohio.
Cyst nematodes were identified from an Ohio bentgrass golf course sample submitted to the Purdue Plant and Pest Diagnostic Lab and the Purdue Nematology Lab for diagnosis in 2016. The affected turf had irregular patches of thinning, translucent leaves, and reduced root systems. Cysts were collected from the infested soil by the modified Jenkins sugar flotation method. Based on the results of morphological observations and DNA sequence analysis, the nematodes were identified as H. iri [Heterodera avenae]. Reproduction on bentgrass was demonstrated by inoculating 1 000 viable eggs onto 5-week-old bentgrass seedlings grown in 4-in. pots (n=2) of sterilized sand at 23°C. A pot of noninoculated seedlings served as a control. Sixty days after inoculation, cysts were harvested from the plants, and extraction produced ∼2 000 eggs, demonstrating multiplication on this host. No cysts were found on the control plants. This detection represents a new record of H. iri in Ohio.