Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

First report of Grapevine syrah virus-1 in Grapevines in China.

Abstract

In 2017, a sample from cultivar SO4 grapevine rootstock (Vitis berlandieri Ă— Vitis riparia) exhibiting chlorotic mottling and deformation was collected in Liaoning province in China. Results of RNA sequencing and BLAST analysis revealed four viruses and one viroid: Grapevine Syrah virus-1 (GSyV-1), Grapevine leafroll-associated virus 3, Grapevine virus A, Grapevine virus and Hop stunt viroid. Fifty-two contigs with lengths of 34 to 257 bp (53% coverage) showed 90.48 to 96.28% nucleotide (nt) identity with the genome sequence of a USA isolate of GSyV-1 (FJ436028). To further confirm the infection by GSyV-1, PCR assay and sequence comparisons were performed. To evaluate the incidence of GSyV-1 in China, 90 samples of different grapevine cultivars were collected from 10 different provinces and regions and were tested by reverse transcription PCR. The results showed that 4.4% (4 of 90) of the samples tested positive for GSyV-1. GSyV-1 was found in wine grape cultivars Cabernet Gernischt (2), Vidal blanc (1), and Marselan (1). This is thought to be the first report of GSyV-1 in China.