First report of Clover yellow mosaic virus in postentry quarantine Tulipa gesneriana plants imported from the United States.
In October 2016, a plant of Tulipa gesneriana cultivar Ile de France, showing chlorotic streaks on leaves and colour breaking on flowers, was received at the Plant Health and Environment Laboratory, Auckland, New Zealand (NZ). This plant originated from bulbs imported from the USA and was being held in a postentry quarantine facility in Southland, NZ. Electron microscopy of a crude sap preparation from the symptomatic leaf tissue revealed the presence of potex-like filamentous virus particles. Based on the results of reverse-transcription PCR assays and nucleotide sequence analysis, the causal agent was identified as Clover yellow mosaic virus (ClYMV). This virus has been reported to occur naturally in clover, lucerne, common chickweed, peas, broad beans, apple, and verbena, although only a few sequences of broad bean and verbena isolates are available in GenBank to date. This is thought to be the first finding of ClYMV in tulip in the world.