Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Regeneration of leaves of noni.

Abstract

Using noni (Morinda citrifolia) leaves as explant for culture in vitro on 3% MS medium with different types and concentrations of plant hormones, two kinds of in vitro regeneration modes were built. Mode I: the callus was induced first, and then the adventitious roots and buds were induced; Mode II: the adventitious roots were induced, and then the buds were induced directly. The results showed that the optimal medium of generating callus by noni leaves in Mode I was MS+0.1 mg.L-1 6-BA+2.0 mg.L-1 2,4-D; the optimal medium that induced leaf callus to generate adventitious roots and buds was MS+1.0 mg.L-1 6-BA+0.4 mg.L-1 NAA or MS+2.0 mg.L-1 6-BA+0.4 mg.L-1 NAA, Thereinto, the solution of MS+1.0 mg.L-1 6-BA+0.4 mg.L-1 NAA made the rooting time earlier, about 10 d, and its root system was more developed. Instead, the solution of MS+2.0 mg.L-1 6-BA+0.4 mg.L-1 NAA made it 15 d. The optimal medium that induced leaves to generate roots and buds in mode II was MS+1.0 mg.L-1 6-BA+0.4 mg.L-1 NAA. Cutting and transplanting the seedlings regenerated in vitro from model I and Model II into MS+0.2 mg.L-1 NAA medium to induce rooting. Getting the differentiation of adventitious root about 15 d and complete plant 45 d. This study provides useful references for the breeding and the application of genetic transformation technique in noni.