Invasive Species Compendium

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Abstract Full Text

Identification and distribution of Fusarium oxysporum f. sp. cubense physiological race in Guangxi.

Abstract

Objective: The present experiment was conducted to investigate type and distribution of Fusarium oxysporum f. sp. cubense physiological races in banana growing areas of Guangxi, in order to lay foundation for developing disease-resistant breeding techniques and taking comprehensive measures to control disease. Method: A total of 38 diseased samples with the typical symptom of banana wilt were collected from banana-producing areas of Guangxi, and pathogens were isolated through conventional tissue isolation method. Types of F. oxysporum f. sp. cubense physiological races were confirmed based on morphological features and PCR results of specific primers. Result: The results showed that, 35 strains of F. oxysporum were isolated from 38 diseased samples, 34 of which were identified as F. oxysporum f. sp. cubense. The detection rate of pathogen was 89.47%. Among 34 strains of F. oxysporum f. sp. cubense, 9 strains were F. oxysporum f. sp. cubense race 1 (Foc1), 25 strains were F. oxysporum f. sp. cubense race 4(Foc4). The detection rates of Foc1 and Foc4 were 26.47% and 73.53%, respectively. Foc1 were distributed in banana-producing areas. Foc4 species belonged to tropical species race 4(TR4), was mainly distributed in banana-producing areas of mid-eastern and southeastern Guangxi. Conclusion: TR4 is dominant population of F. oxysporum f. sp. cubense physiological races in Guangxi, so it should be choosed as target pathogen for breeding disease-resistant varieties. The areas suitable for bananas growth in west and north Guangxi should preferentially be elect as new banana-producing areas.