First report of Maize dwarf mosaic virus in Johnsongrass in Oklahoma.
During a survey in the summer of 2016, Johnsongrass (Sorghum halepense) plants showing typical virus-like symptoms including streaking and mottling were collected in Muskogee County in Oklahoma, USA. Four plants were randomly selected, uprooted from the field, and grown in pots in a growth chamber. Based on particle size, reverse transcription-polymerase chain reaction, BLAST analysis of the nucleotide sequences and dot-immunobinding assay, the causal agent was identified as Maize dwarf mosaic virus (MDMV). Although the presence of MDMV has been confirmed in maize in Oklahoma, this is thought to be the first report of MDMV in Johnsongrass in Oklahoma.