First report of Chickpea chlorotic dwarf virus infecting cucumber in Pakistan.
Field surveys were conducted during 2012 to 2013 to determine the incidence of cucurbit-infecting viruses in Punjab Province, Pakistan. Virus-like symptoms were observed in cucumber (Cucumis sativus) plants, including leaf curling, mosaic, and necrotic spots, in commercial fields in the Vehari district of Pakistan. Disease incidence ranged between 20 to 30%. Both whiteflies and leafhoppers were observed to colonize cucumber plants. Leaf samples were collected from seven symptomatic plants, and total DNA was isolated using CTAB method. DNA sequence analysis revealed the presence of Chickpea chlorotic dwarf virus (CpCDV), which was confirmed by Southern blot hybridization. CpCDV has also been reported infecting tomato plants in Pakistan. This is thought to be the first report of CpCDV infecting cucumber worldwide.