First report of a 16SrII group related phytoplasma associated with witches' broom of Cupressus sempervirens var. horizontalis in Iran.
In 2014-2015, cypress (Cupressus sempervirens var. horizontalis) trees with witches' broom, abnormal shoot proliferation and dieback on one side of the trees, were observed in Tehran, Iran. Diseased shoots were collected from 4 affected trees at 2 locations and analysed in the laboratory. The causal pathogen was identified as a 16SrII-D type phytoplasma based on various molecular analyses. This is thought to be the first report of the natural occurrence of a phytoplasma associated with a witches' broom disease of C. sempervirens var. horizontalis in Iran.