Parasitization of the common bean (Phaseolus vulgaris) by Meloidogyne ethiopica in southern Brazil.
During March 2014, common bean plants (Phaseolus vulgaris cv. IPR Gralha) exhibiting symptoms of stunting, leaf yellowing, and numerous galls caused by root-knot nematode were detected and sampled in Caiçara County, Northwest Rio Grande do Sul State, Brazil. The identification of Meloidogyne was conducted using esterase phenotypes (n=20), morphological analyses of females by perineal patterns (n=20), morphological measurement of second-stage juveniles (J2) (n=20), and amplification of the D2-D3 fragment of the 28S rRNA gene. In addition, the root and soil samples were processed to determine the population density of nematode eggs and juveniles. Greenhouse tests were also conducted to confirm parasitization of common bean by M. ethiopica. This is thought to be the first report of M. ethiopica parasitizing common bean roots in Brazil.