Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

First report of anthracnose in Baishao (Radix paeoniae alba) caused by Colletotrichum gloeosporioides in China.

Abstract

Baishao (Radix paeoniae alba [Paeonia lactiflora]) is one of the most important Chinese medicine plants in Zhejiang and Anhui provinces in China. In May 2013, sporadic occurrence of leaf damage with disease incidence of ≤10% by anthracnose in baishao was observed in Pan'an, Zhejiang Province, a well-known traditional region for baishao plantings with an area of ∼200 ha. During May 2016, ∼35% of fields in Zhejiang Province were affected by anthracnose disease. Symptoms were extensive, with lesions on stems and leaves that began as brown to reddish-brown spots and then coalesced into larger lesions. Under wet conditions, orange masses of conidia were produced in acervuli in the centre of lesions. A total of 26 infected tissue samples were surface sterilized with 1.5% sodium hypochlorite for 2.5 min, rinsed twice in sterilized water, plated on 2% potato dextrose agar, and incubated at 25°C in the dark for one week. Based on morphological and cultural characteristics, molecular data (PCR and BLAST sequencing analyses of the partial ITS rDNA region, actin, β-tubulin and glyceraldehyde 3-phosphate dehydrogenase (phosphorylating)) and pathogenicity tests, the causal agent was identified as Colletotrichum gloeosporioides [Glomerella cingulata]. This is thought to be the first report of anthracnose in baishao.