Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract Full Text

Procedure for the identification of sexual differentiation genes in Hemichromis guttatus females.


The identification of sexual DNA markers has served many purposes, from basic knowledge on sexual development and differentiation, to the assessment of sex reversion of hormonal exposed fish. In the present study several candidate genes were analyzed to be validated as sexual markers for the cichlid Hemichromis guttatus a well-known invasive species whose genome or any genetic information is not yet known and that has important ecological impacts in a natural protected area characterized by several endemisms. A way to eradicate invasive species is the Trojan Y-Chromosome hormonal strategy in which males with two Y chromosomes are breed with females of the invasive population to lead to the decline or disappearance of the invasive population. The identification of candidate marker genes in the cichlid genome was confirmed by PCR essays using genomic DNA, and the expression of these same genes using mRNA extractions. Genomic material was extracted from gonadal tissue from H. guttatus specimens, regardless of their sex, size or age. Four candidate genes have been selected for sexual identification of phenotypically differentiated females, and thus primers for the orthologues genes: Foxl2a, Figla, Wnt4a and Ctnnb1b have been obtained. All genes have been successfully amplified using both nucleic acids.