Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

First report of a new bacterial leaf blight of rice caused by Pantoea ananatis and Pantoea stewartii in Togo.

Abstract

In 2013 and 2014, surveys were carried out in the main rice-growing regions of Togo (Kovié and Kpalimé) to evaluate the prevalence of plant-pathogenic bacteria, such as Xanthomonas oryzae. Symptomatic rice leaves showing characteristics of bacterial leaf blight, as typically caused by X. oryzae pv. oryzae, were collected, surface-sterilized, and macerated in sterile water. Upon plating on semiselective peptone-sucrose-agar medium, yellowish colonies of bacteria were isolated. Similar bacterial colonies were isolated from rice grains. Sequence comparisons of the obtained 603-bp DNA fragments revealed that the sequences of the leaf isolate ARC60 (GenBank accession no. KX385187) and of the seed isolate ARC651 (KX342014) were 98% identical to the corresponding gyrB gene fragment from the Pantoea ananatis type strain LMG 2665 (JMJJ01000009). The sequences of the leaf isolate ARC229 (KX342015) and of the seed isolate ARC646 (KX342016) were 99% and 100% identical to the corresponding gyrB gene fragment from the P. stewartii type strain LMG 2632 (JPKO01000005), respectively. Using species-specific gyrB primers for Pantoea, 91 isolates were confirmed as P. ananatis or P. stewartii from 12 localities. Pathogenicity tests were carried out to further confirm the identity of the pathogens. Based on the results, it is concluded that the bacteria that were isolated from rice leaves and grains in Togo were P. ananatis and P. stewartii. This is thought to be the first report of leaf blight of rice caused by species of Pantoea in Togo.