First report of Cladosporium oxysporum causing leaf spot of Rosa indica in Pakistan.
In December 2014, necrotic leaf spots were observed on >50 rose (Rosa indica [R. chinensis]) plants in a private garden at Lahore, Pakistan, all showing the same symptoms. The infected plants were 1-2 years old and on an average 90% of the leaves were covered with 2-5 mm circular black spots, with irregular margins without chlorosis around the margins. Initially, infected leaves remained attached to the plants but dropped when the spots coalesced and covered more than 80% leaf area. No symptoms were observed on flowers. Based on morphological characteristics, sequence analysis of the DNA internal transcribed spacer region and pathogenicity test, the causal agent was identified as Cladosporium oxysporum. This is thought to be the first report of R. indica leaf spot caused by C. oxysporum in Pakistan.