Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

First report of Tobacco rattle virus infecting Chinese herbaceous peony (Paeonia lactiflora Pall.) in China.

Abstract

In the summer of 2015, virus-like symptoms such as leaf yellowing, mottling, and mosaic were observed on Chinese herbaceous peony (Paeonia lactiflora) plants in Yangzhou City, Jiangsu Province, China. During the spring of 2016, a total of 26 herbaceous peony samples were collected in Yangzhou City, and total RNA were extracted. Using RT-PCR, Tobacco rattle virus (TRV) was detected in 19 (73.1%) samples. However, TRV was only detected in 17 (65.4%) of the samples using ELISA, possibly due to low virus concentration in the plant. The purified amplicons were direct sequenced using an automated DNA sequencer, and BLASTn analysis of these sequences revealed 100% nucleotide identity with a TRV isolate (Accession No. AF406990). This is thought to be the first report of TRV in Chinese herbaceous peony in China.