Invasive Species Compendium

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Effect of aqueous extract of Galinsoga parviflora Cav. on guard cells of Vicia faba L.

Abstract

To explore the allelopathy mechanism of an invasive plant (Galinsoga parviflora Cav.) in China, the leaf guard cells apoptosis and signal regulation of broad bean (Vicia faba L.) epidermis were treated with the aqueous extracts of G. parviflora were studied. The results showed that when broad bean was exposed to the aqueous extracts, its guard cell viabilities reduced, the typical features of apoptosis including nuclear pyknotic, malposition, stretch and degradation were observed, and these effects were concentration-dependent. The mortality rates and the abnormal nuclei rates were 90.53% and 87.77%, respectively while the aqueous extracts concentration were 0.05 g/mL. However, the mortality rates significantly decreased when the strips exposed to the aqueous extracts combined with different concentrations of plasma membrane Ca2+ channel blocker LaCl3 or nitrite reductase inhibitor NaN3 or antioxidant ascorbic acid (AsA) or Caspase inhibitor (Z-VAD-FMK), which were 19.44%, 16.12%, 50.08%, 23.67%. These results suggested that the apoptosis of broad bean induced by the allelochemical stress of G. parviflora was associated with Ca2+, NO and ROS.