First report of the root-lesion nematode Pratylenchus neglectus on wheat (Triticum aestivum) in North Dakota.
In August 2015, a soil sample was collected from a harvested wheat field in Walsh County, North Dakota, USA, and was found to contain 1044 root-lesion nematodes/kg of soil using the sugar centrifugal flotation method. In October 2015, four soil samples were collected from the same field and had root-lesion nematodes ranging from 125 to 1000/kg soil. One soil sample with 500 lesion nematodes/kg soil was used to inoculate hard red spring wheat cultivars Glenn and Faller. After 10 weeks of growth in a greenhouse room maintained at 22°C, roots were harvested and washed and light brown lesions were observed on lateral roots. The washed roots were cut into 1-cm segments for nematode extraction using the Whitehead tray method. After 48 h, lesion nematodes were recovered from the root tissues. Averages of 24 and 20 root-lesion nematodes per gram were found in the roots of Glenn and Faller, respectively. Nematodes from soil and wheat roots were examined morphologically and molecularly and were identified as Pratylenchus neglectus. This is thought to be the first report of P. neglectus on wheat in North Dakota, USA.