Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Molecular identification of puffer fish Lagocephalus sceleratus (Gmelin, 1789) and Lagocephalus spadiceus (Richardson, 1845) from eastern Mediterranean, Turkey.

Abstract

The Mediterranean Sea is home to numerous invasive marine species. The Suez Canal is considered as the major route of migrating Indo-Pacific species known as Lessepsian species, from the Red Sea into the Mediterranean. One of those species Lagocephalus sceleratus and Lagocephalus spadiceus has serious impact on the ecosystem, fisheries and human health within a decade. DNA based methods are frequently preferred and reliable techniques in species identification studies. The 16S rRNA genes and cytochrome b genes are relatively conserved mitochondrial genes, used for this purpose. 16S rRNA gene primers and cytochrome b gene specific primers were used to identify Turkish L. sceleratus and L. spadiceus specimens from Gökova Bay via PCR analysis. Obtained gene sequence deposited in GenBank database. Consequently, it was obtained that cytochrome b gene sequence is more useful than 16S rRNA gene region for identification of L. spadiceus samples while both gene region give reasonable results for L. sceleratus species.