Incidence effect by nano Spinosad of the invasive tomato leafminer, Tuta absoluta Meyrick, (Lepidoptera: Gelechiidae) under laboratory and field condition.
The tomato leafminer, Tuta absoluta Meyrick, (Lepidoptera: Gelechiidae) controlled by the biocontrol agent spinosad and its nano particles under laboratory conditions, the LC50s of the T. absoluta Meyrick, (Lepidoptera: Gelechiidae) give, 56, 68, 109, 131, 149, 173 and 175 ppm after Newly hatched larvae, 1st larval instars, 2nd larval instars, 3rd larval instars, 4th larval instars, Adult males and adult females, respectively. Under laboratory conditions, the effect of the nano-Spinosad calculated that, 20, 22, 32, 40, 57 and 59 for the T. absoluta for the corresponding stages, respectively. The effect of the tested pathogen under green house effect give a resulted, show, the nano Spinosad gave a significant decrease of infestations after 20, 50, 0 and 120 days after the post application of green house application and recoded, 1.1±3.2, 5.2±1.1, 9.2±6.1, and 10.0±4.1 individuals. When the totato plots treated with spinosad, after the corresponding days the infestation individuals recorded 11.0±5.1, 18.6±3.1, 31.5±1.8 and 57.4±2.9 individuals as compared to 39.2±6.7, 68.1±5.7, 78.8±9.1 and 99±7.9 individuals in the control. Field condition experiments show that, the amount of the harvest Tomato plant Winter Variety Platenium-5043 in the fields of Fayoum governorates, after nano Spinosad, the weight of tomatoes significantly increased to 16100±21.60 and 16700±31.80 kg/feddan as compared to 15000±41.11 kg/fedan in the control. While in Tanta governorates, the tomatoes crop weight 16231±26.40 and 17200±26.70 kg/feddan in the plots treated with Spinosad and nano Spinosad, respectively as compared to 14900±51.60 kg/feddan in the control.