Efficient micropropagation by multiple shoot induction through recurrent regeneration in Celosia cristata L.
An investigation was carried out to study the effect of various factors on the regeneration of Celosia cristata L. The 6-benzyladenine (BAP) was more effective than kinetin (Kn) for shoot regeneration. The presence of 2,4-dichlorophenoxy acetic acid (2,4-D) or 1-naphthalene acetic acid (NAA) at low concentration increased the number of shoots per explant. Similarly, salt and sugar composition of medium also affected the in vitro regeneration. Subculture of explants up to 8th generations remarkably increased regeneration efficiency. Up to 1,434 shoots, with an average of 239 shoots per explant, could be regenerated by subculture of the explants over the medium containing 6.6 µM BAP and 0.5 µM NAA. The induced microshoots were rooted in 1/8th MS basal medium. It was concluded that shoot tips derived from 12-d-old seedling were the best explant for micropropagation through recurrent regeneration when they were cultured over MS medium containing 0.5 µM NAA and 6.6 µM BAP.