First report of Leek yellow stripe virus, Shallot latent virus, and Onion yellow dwarf virus in garlic from Ecuador.
Seventy-one garlic leaves were sampled from symptomatic plants showing mild to severe chlorotic streaking and leaf curling in the fields of an experimental farm in Tumbaco (Pichincha Province). These samples corresponded to clove seeds collected during April 2011 from commercial cultivars; 13 from Pungalá (Chimborazo Province), 8 from Cebadas (Chimborazo Province), 3 from Pelileo (Tungurahua Province), and 47 from local markets in Quito (Pichincha Province). Results of RT-PCR and nucleotide sequence analysis revealed coinfection of the most important viruses from the genera Potyvirus and Carlavirus, namely Leek yellow stripe virus, Shallot latent virus and Onion yellow dwarf virus, necessitating the evaluation and management of effects on garlic production. This is thought to be the first report of these viruses in garlic from Ecuador.