First report of Tomato leaf curl New Delhi virus on opium poppy (Papaver somniferum) in India.
During a survey in 2012, severe curling of P. somniferum leaves was observed in breeding plots of CSIR-National Botanical Research Institute, Lucknow, Uttar pradesh, India. Whiteflies (Bemisia tabaci) were also noticed on the poppy plants. Based on typical begomovirus-like symptoms and the whitefly population, a begomovirus infection was suspected. Results of PCR, rolling circle amplification and analysis of the nucleotide sequences revealed the causal agent as Tomato leaf curl new Delhi virus (ToLCNDV). Nucleic acid spot hybridization tests were carried out, which resulted in positive signals from 19 out of 23 samples, indicating prevalence of ToLCNDV in 82.6% P. somniferum plants. Papaver spp. are described in the literature as natural hosts of Turnip mosaic virus, Bean yellow mosaic virus, Beet yellows virus and Beet mosaic virus. However, association of begomovirus with P. somniferum has not been hitherto reported; this paper reports ToLCNDV infection of P. somniferum for the first time.