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Abstract

In vitro evaluation of fungicides, biocontrol agents and botanicals for their bioefficacy against root rot of acid lime (Citrus aurantifolia Swingle) in northern Karnataka.

Abstract

This paper presents the results of a study conducted in vitro to determine the effective fungicides, biological control agents (BCAs) and botanicals against the root rot (caused by Fusarium solani [Haematonectria haematococca]) of acid lime. Seven systemic fungicides, i.e. carbendazim, propiconazole, hexaconazole, thiophanate-methyl, carboxin+thiram, metalaxyl MZ and fosetyl-Al [fosetyl] (at 0.05, 0.1 and 0.15%), and 5 non-systemic fungicides, i.e. mancozeb, captan, copper oxychloride, Bordeaux mixture and chlorothalonil (at 0.1, 0.2 and 0.25%), were assayed following the poisoned food technique. Nine BCAs, namely Trichoderma harzianum, T. viride, T. virens, T. asperellum, T. harzianum (local), Paecilomyces lilacinus, Verticillium lecani [Lecanicillium lecanii], Pseudomonas fluorescens and Bacillus subtilis, were tested using the dual culture technique. The botanicals used at 5 and 10% were Allium cepa, A. sativum, Bougainvillea spectabilis, Pongamia pinnata, Tridax procumbens, Lantana camara, Duranta repens [D. erecta], Azadirachta indica, Parthenium hysterophorus and Capsicum annuum, using the poisoned food technique. The results indicated that carbendazim (96.83% inhibition) and chlorothalonil (50.41% inhibition) among the systemic and non-systemic fungicides, respectively, were significantly the most effective against the root rot pathogen. Also, T. harzianum and Allium sativum at 10% among the BCAs and botanicals, respectively, were significantly effective in inhibiting (60.37%) the mycelial growth of F. solani.